The reaction mixtures were incubated for
30 min in dark room and the absorbance at 517 nm was read
against methanol as a blank. Standard ascorbic acid was
Noor Azwani Mohd Rasidek et al./Asian Pac J Trop Biomed 2016; 6(1): 55–59 56
prepared at 0.1–10 mg/mL and the linear regression for sample
references was generated.