TABLE 1. EXAMPLES OF THE MOLECULAR UV/VIS ANALYSIS OF CLINICAL SAMPLES [10]
Analyte Method λ (nm)
serum barbiturates extract into CHCl3 to isolate from interferents and then extract into 0.45 M NaOH 260
protein-bound
iodine
decompose protein to release iodide, which catalyzes redox reaction between Ce3+
and As3+; forms yellow colored Ce4+
420
total serum protein react with NaOH and Cu2+; forms blue-violet complex 540
serum cholesterol
react with Fe3+ in presence of isopropanol, acetic acid, and H2SO4; forms blueviolet
complex
540
glucose react with o-toludine at 100°C; forms blue-green complex 630
uric acid react with phosphotungstic acid; forms tungsten blue 710