2.2. Mass change of frozen bread
The total-mass change of frozen bread was monitored with whole (triplicates) and half-cut (duplicates) samples.
At certain storage time points, the plastic bags containing the frozen bread
were taken out of the freezer.
The bread was then removed from the bags and, after the ice on the bread surface was gently shaken off, placed into new dry plastic bags.
This prevented the ice in the original bags from thawing back into the bread and minimized the condensation of environmental moisture onto the bread surface while it was still cold.
The dry-surfaced frozen bread samples were hence weighed. Subsequently the samples were put in another set of new plastic bags and placed back into the freezer for storage until the next evaluation.
The same procedure was repeated daily during the first week and, thereafter, at longer intervals over the storage up to w4 months.