Centrifugaldamagehasbeenknowntoalte

Centrifugaldamagehasbeenknowntoalterbacterialcellsurfacepropertiesandinteriorstructures,includingDNA.Veryfew studiesexistonbacterialdamagecausedbycentrifugationbecauseofthedifficultyinrelatingcentrifugationspeedandcontainergeometrytothedamagecaused.Here,weprovideasimple,versatilemethodofanalysisfordescribingthecompactionof bacteriaduringcentrifugationbasedonaproposedcentrifugationcoefficient,C.ValuesofCcanberelatedtodifferentbacterial cellsurfaceproperties.ChangingthegeometryofthecentrifugationcontainerorcentrifugationspeedschangedthevalueofC significantly.InitialdepositionratesofStaphylococcusaureusATCC12600toaglasssurfacedecayedexponentiallyfrom4,217 to1,478cm
2 s
1 withincreasingC,whiletheproportionofstaphylococciwithazetapotentialofaround
15mVdecreased from97to58%.Thesesurface-sensitiveparameterswereusedindependentlytoderiveacriticalcentrifugationcoefficient (0.040),abovewhichcentrifugationwasconsideredtoimpacttheoutcomeofsurface-sensitiveexperimentsduetocellsurface damage.Thecriticalcentrifugationcoefficientcouldsuccessfullypredictstaphylococcalcellsurfacedamage,i.e.,asignificant changeininitialdepositionrateorzetapotentialdistribution,in84%ofallcasesincludedhere,whereasthecentrifugation speedcouldpredictdamageinonly58%ofallcases.Moreover,controllingthecentrifugationcoefficientwithinnarrowlimits overaseriesofexperimentsyielded43%smallerstandarddeviationsininitialstaphylococcaldepositionratesthanwithcentrifugationatfixedspeedsforreplicateexperiments.

ผลลัพธ์ (ไทย) 1:

Centrifugaldamagehasbeenknowntoalterbacterialcellsurfacepropertiesandinteriorstructures,includingDNA.Veryfew studiesexistonbacterialdamagecausedbycentrifugationbecauseofthedifficultyinrelatingcentrifugationspeedandcontainergeometrytothedamagecaused ที่นี่ weprovideasimple, versatilemethodofanalysisfordescribingthecompactionof bacteriaduringcentrifugationbasedonaproposedcentrifugationcoefficient,C.ValuesofCcanberelatedtodifferentbacterial cellsurfaceproperties ChangingthegeometryofthecentrifugationcontainerorcentrifugationspeedschangedthevalueofC significantly InitialdepositionratesofStaphylococcusaureusATCC12600toaglasssurfacedecayedexponentiallyfrom4, to1 217, withincreasingC s 1 478 ซม. 2, whiletheproportionofstaphylococciwithazetapotentialofaround 15mVdecreased from97to58% Thesesurface-sensitiveparameterswereusedindependentlytoderiveacriticalcentrifugationcoefficient (0.040), abovewhichcentrifugationwasconsideredtoimpacttheoutcomeofsurface-sensitiveexperimentsduetocellsurface ความเสียหาย Thecriticalcentrifugationcoefficientcouldsuccessfullypredictstaphylococcalcellsurfacedamage,i.e.,asignificant changeininitialdepositionrateorzetapotentialdistribution, in84% ofallcasesincludedhere, whereasthecentrifugation speedcouldpredictdamageinonly58% ofallcases นอกจากนี้ controllingthecentrifugationcoefficientwithinnarrowlimits overaseriesofexperimentsyielded43% smallerstandarddeviationsininitialstaphylococcaldepositionratesthanwithcentrifugationatfixedspeedsforreplicateexperiments

ผลลัพธ์ (ไทย) 3:

centrifugaldamagehasbeenknowntoalterbacterialcellsurfacepropertiesandinteriorstructures includingdna.veryfew , studiesexistonbacterialdamagecausedbycentrifugationbecauseofthedif จึง cultyinrelatingcentrifugationspeedandcontainergeometrytothedamagecaused ที่นี่ weprovideasimple versatilemethodofanalysisfordescribingthecompactionof , bacteriaduringcentrifugationbasedonaproposedcentrifugationcoef จึง cient , C .valuesofccanberelatedtodifferentbacterial cellsurfaceproperties.changingthegeometryofthecentrifugationcontainerorcentrifugationspeedschangedthevalueofc signi จึง cantly.initialdepositionratesofstaphylococcusaureusatcc12600toaglasssurfacedecayedexponentiallyfrom4217 to1478cm
2 s
1 withincreasingc whiletheproportionofstaphylococciwithazetapotentialofaround
, 15mvdecreased from97to58 %thesesurface sensitiveparameterswereusedindependentlytoderiveacriticalcentrifugationcoef จึง cient ( 0.040 ) , ความเสียหาย sensitiveexperimentsduetocellsurface abovewhichcentrifugationwasconsideredtoimpacttheoutcomeofsurface . thecriticalcentrifugationcoef จึง cientcouldsuccessfullypredictstaphylococcalcellsurfacedamage ) จึงไม่สามารถ changeininitialdepositionrateorzetapotentialdistribution asigni ,in84 % ofallcasesincludedhere whereasthecentrifugation , speedcouldpredictdamageinonly58 % ofallcases นอกจากนี้ controllingthecentrifugationcoef จึง cientwithinnarrowlimits overaseriesofexperimentsyielded43 % smallerstandarddeviationsininitialstaphylococcaldepositionratesthanwithcentrifugationat จึง xedspeedsforreplicateexperiments .