Lactobacillus rhamnosus (ATCC 53103) was provided in a freeze-dried form. The viability of the
freeze-dried bacteria, 1.051013 CFU/g, was determined by flow cytometry [11] and by plate counting on
MRS agar (de Man, Rogosa and Sharpe; MRS, Merck, Darmstadt). Commercial dry feed served as a basis
in which the various concentrations of bacteria were added, in 20 ml of sunflower oil, to commercial dry
feed followed by mixing with a blender. The amount of L. rhamnosus in each feed was determined by plate
counting on MRS by homogenising 1 g of feed in 9 ml sterile PBS, pH 7.4 and spreading appropriate
dilutions from 101 to 1012 on MRS plates and the plates were incubated at 37 (C for two days. Feeds
were stored at 4 (C until use. The control feed was only supplemented with sunflower oil.
Lactobacillus rhamnosus (ATCC 53103) was provided in a freeze-dried form. The viability of thefreeze-dried bacteria, 1.051013 CFU/g, was determined by flow cytometry [11] and by plate counting onMRS agar (de Man, Rogosa and Sharpe; MRS, Merck, Darmstadt). Commercial dry feed served as a basisin which the various concentrations of bacteria were added, in 20 ml of sunflower oil, to commercial dryfeed followed by mixing with a blender. The amount of L. rhamnosus in each feed was determined by platecounting on MRS by homogenising 1 g of feed in 9 ml sterile PBS, pH 7.4 and spreading appropriatedilutions from 101 to 1012 on MRS plates and the plates were incubated at 37 (C for two days. Feedswere stored at 4 (C until use. The control feed was only supplemented with sunflower oil.
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