When Fomitopsis palustris TYP-0507 and Antrodia xantha Shiga-1F were independently cultivated with CuSO4-treated Japanese cedar (C. japonica) blocks, both fungi transferred copper from inside the blocks to the interface between the wood surface and the fungal mat within 2 weeks of cultivation, before significant wood weight loss occurred. Fomitopsis palustris TYP-0507 further transferred copper to mycelia located far from the wood block. Some of the transferred copper was complexed with oxalate to produce moolooite at the interface. This copper transfer and precipitation allows copper to be removed by brushing off the moolooite and fungal mycelia. The rapidity with which both fungi removed copper from treated wood makes this method practical for the bioremediation of heavy metals.