When applying AgNPs in biological systems, their bioavailabili-
ty and cytotoxicity are governed by colloidal stability in respective
environment, which may be influenced by several variables, such
as pH, ionic strength, and the type of electrolyte present (El
Badawy et al., 2010; Römer et al., 2011; Prathna et al., 2011). A
number of studies dealing with the aggregation of nanoparticles in
various environments has been conducted and reported however, only limited
information can be found in literature dealing with the influence of
the above listed variables on the changes of particles used for
biological studies.