Resistance to gastrointestinal conditions is a requirement for bacteria to be considered probiotics. In this
work, we tested the resistance of six different Lactobacillus strains and the effect of carbon source to
four different gastrointestinal conditions: presence of a-amylase, pancreatin, bile extract and low pH.
Novel galactooligosaccharides synthesized from lactulose (GOS-Lu) as well as commercial galactooligosaccharides
synthesized from lactose (GOS-La) and lactulose were used as carbon sources and
compared with glucose. In general, all strains grew in all carbon sources, although after 24 h of
fermentation the population of all Lactobacillus strains was higher for both types of GOS than for glucose
and lactulose. No differences were found among GOS-Lu and GOS-La. a-amylase and pancreatin resistance
was retained at all times for all strains. However, a dependence on carbon source and Lactobacillus
strain was observed for bile extract and low pH resistance. High hydrophobicity was found for all strains
with GOS-Lu when compared with other carbon sources. However, concentrations of lactic and acetic
acids were higher in glucose and lactulose than GOS-Lu and GOS-La. These results show that the
resistance to gastrointestinal conditions and hydrophobicity is directly related with the carbon source
and Lactobacillus strains. In this sense, the use of prebiotics as GOS and lactulose could be an excellent
alternative to monosaccharides to support growth of probiotic Lactobacillus strains and improve their
survival through the gastrointestinal tract.
Resistance to gastrointestinal conditions is a requirement for bacteria to be considered probiotics. In thiswork, we tested the resistance of six different Lactobacillus strains and the effect of carbon source tofour different gastrointestinal conditions: presence of a-amylase, pancreatin, bile extract and low pH.Novel galactooligosaccharides synthesized from lactulose (GOS-Lu) as well as commercial galactooligosaccharidessynthesized from lactose (GOS-La) and lactulose were used as carbon sources andcompared with glucose. In general, all strains grew in all carbon sources, although after 24 h offermentation the population of all Lactobacillus strains was higher for both types of GOS than for glucoseand lactulose. No differences were found among GOS-Lu and GOS-La. a-amylase and pancreatin resistancewas retained at all times for all strains. However, a dependence on carbon source and Lactobacillusstrain was observed for bile extract and low pH resistance. High hydrophobicity was found for all strainswith GOS-Lu when compared with other carbon sources. However, concentrations of lactic and aceticacids were higher in glucose and lactulose than GOS-Lu and GOS-La. These results show that theresistance to gastrointestinal conditions and hydrophobicity is directly related with the carbon sourceand Lactobacillus strains. In this sense, the use of prebiotics as GOS and lactulose could be an excellentทาง monosaccharides เพื่อสนับสนุนการเจริญเติบโตของโปรไบโอติกส์แลคโตบาซิลลัสสายพันธุ์ และปรับปรุงตนการอยู่รอดผ่านระบบทางเดิน
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