where t = growth period during which the maximum dry cellmass was obtained. All experiments were carried out in triplicateand the values are presented as mean ± standard deviation (SD).Different concentration of sucrose (0.3%, 1%, 1.5% and 3%) in thebasal liquid medium was tested to study the effect of sucrose on cellgrowth. The time course of absorbance of the culture was read usinga UV–spectrophotometer (UV-2450, Shimadzu) and plotted to forma growth curve to be fitted to the Michaelis–Menten equation andKmand Vmaxvalues were calculated.