Fig. 3b shows the time course for growth of C. rugosa during lipase production using different concentrations
of fatty acids residues. The maximum cell mass obtained was 1.4 g/l by using 1.5% fatty acids residues. Also, presence of the above mentioned materials have an adverse effect on the production rate of the enzyme. Fig. 3c shows the time course of lipase production using 1% fatty acids residues, where the results showed that at the maximum activity of 7 U/ml after 24 h, the protein content was 0.15 mg/ml.