Esterase solution
10 mg (20 mL in 1mMsodium phosphate buffer pH 7.2)was added to
each well and reactions were followed by measuring the decrease
in absorbance at 410 nm for 2 h at 37 C in a Synergy HT BioTek
microplate spectrophotometer. Blanks without enzyme were carried
out for each substrate and data were collected in triplicate and
the average activities were quantified