Shoot tip or Apical meristems that are of 0.5mm in length isolated
from growing tips of current shoots of Psoralea corylifolia were used in the experiment as explants. Explants were washed under running tap water to remove dust particles for 30 min, and treated with liquid detergent for 10 min, and rinsed three times with distilled water. Then explant treated with an antifungal agent (Bavistin) for 1 hours and the again rinsed three times with distilled water. Further, sterilization treatments were done under a laminar-flow chamber. The explants were then disinfected with 0.1% (w/v) mercuric chloride for 3 minutes under aseptic conditions. After this these explants were then thoroughly washed 3-4 times with sterilized double distilled water to remove the traces of mercuric chloride now the explants is ready for inoculation on required medium. Shoot tip prepared from twenty day old in vitro raised seedlings were also used as explant. Shoot tip was cut from the top measuring 1-2 cm inside the laminar air flow chamber.