SCC1 therefore
displays four sub-populations when exposed to OJ (Supplemental
Fig. 2): A healthy population (retaining GFP fluorescence and not staining
with PI); a dead population (having lost GFP fluorescence and staining
with PI); and intermediate between these two populations, two stressed
populations that were viable (defined here as not staining with PI) but
had intermediate or lowgreen fluorescence caused by partial or complete
inactivation of GFP due to decreases in pHi (Supplemental Fig. 2c).