Immunofluorescent localization of Burkholderia cenocepacia AU0728. C57/BL6 mice were infected with B. cenocepacia AU0728. Lungs were removed 24 h (A) or 72 h (B) later, fixed, sectioned, stained for bacteria (red ), and counterstained with 4 ,6-diamidino-2-phenylindole (blue). A, Bacteria localized primarily in lung parenchyma, with few bacteria observed in airway lumen. B, By 72 h after infection, bacteria formed microcolonies in areas of consolidated lung parenchyma (bar, 20 mm).
Acknowledgments We thank Steve Magill for providing soil samples and Tim O’Brien for technical assistance