Cell culture systems
-human mucosal cells
*mucosa cells were mixed with pathogenic cell ( E. coli coli)
*rotated for 30 min at room temp.
*after separation, the bacteria was counting
(stain with gential violet)
-HeLa cells
*test the invasive potential of intestinal pathogen
*inoculated monolayer of cells with test culture suspension
*after incubation, the cell was fixed and stained for viewing under light microscopy