The volume-surface mean particle diameters (d32) remained constant as the salt concentration was increased from 0 to 250 mM NaCl (0.21±0.01 μm for LMW–FG and 0.52 ± 0.01 μm for HMW–FG). In addition, d43 was relatively small at all NaCl concentrations studied, although the values were slightly increased at >50 mM NaCl ( Table 1). Compared with d32 (which is more sensitive to the presence of small particles), d43 (which is more sensitive to the presence of large particles) had a relatively high standard deviation, which could be attributed to the sampling problem associated with the preparation of diluted emulsions (see Section 2.11) because optical microscopy showed there was a very small population of big oil droplets present intermittently in the diluted ones collected from the lower portion of the separatory funnel (data not shown). No significant difference was observed in the creaming stability of emulsions containing different salt concentrations (0–250 mM NaCl), however, the LMW–FG emulsions were slightly more stable than the HMW–FG emulsions as discussed earlier. These results suggest that the fish gelatin stabilized emulsions remained relatively stable to droplet aggregation even at high ionic strength where electrostatic interactions should be highly screened. Hence, we can conclude that polymeric steric repulsion (rather than electrostatic repulsion) plays a major role in preventing the droplets from aggregating.
The volume-surface mean particle diameters (d32) remained constant as the salt concentration was increased from 0 to 250 mM NaCl (0.21±0.01 μm for LMW–FG and 0.52 ± 0.01 μm for HMW–FG). In addition, d43 was relatively small at all NaCl concentrations studied, although the values were slightly increased at >50 mM NaCl ( Table 1). Compared with d32 (which is more sensitive to the presence of small particles), d43 (which is more sensitive to the presence of large particles) had a relatively high standard deviation, which could be attributed to the sampling problem associated with the preparation of diluted emulsions (see Section 2.11) because optical microscopy showed there was a very small population of big oil droplets present intermittently in the diluted ones collected from the lower portion of the separatory funnel (data not shown). No significant difference was observed in the creaming stability of emulsions containing different salt concentrations (0–250 mM NaCl), however, the LMW–FG emulsions were slightly more stable than the HMW–FG emulsions as discussed earlier. These results suggest that the fish gelatin stabilized emulsions remained relatively stable to droplet aggregation even at high ionic strength where electrostatic interactions should be highly screened. Hence, we can conclude that polymeric steric repulsion (rather than electrostatic repulsion) plays a major role in preventing the droplets from aggregating.
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