Recent studies on C. perfringens spores have shown that the factors
involved in spore resistance include the: (i) cortex peptidoglycan
(PG) structure; (ii) spore’s core low water content; (iii) high levels of
pyridine-2,6-dicarboxylic acid (dipicolinic acid (DPA)) in the spore
core; and (iv) saturation of spore DNA with a/b-type small, acid
soluble proteins (SASPs). However, it has been shown that during
germination of Bacillus subtilis spores, these factors of resistance are
quickly lost and germinated spores become sensitive to environmental
stress.Interestingly, C. perfringens spores can complete their germination in
less than 20 min in the presence of L-asparagine and Kþ ions (AK)
. Since germinated spores are sensitive to heat and pressure, we hypothesized that formulating foods with certain germinants and then using HPP protocols that include
a spore germination step may lead to an effective strategy to inactivate
bacterial spores. Consequently, the objectives of this
study were to develop: (i) a germination-activation procedure for
C. perfringens spores and (ii) thermal and HPP protocols for the
inactivation of germinated spores of C. perfringens in poultry meat.
Recent studies on C. perfringens spores have shown that the factorsinvolved in spore resistance include the: (i) cortex peptidoglycan(PG) structure; (ii) spore’s core low water content; (iii) high levels ofpyridine-2,6-dicarboxylic acid (dipicolinic acid (DPA)) in the sporecore; and (iv) saturation of spore DNA with a/b-type small, acidsoluble proteins (SASPs). However, it has been shown that duringgermination of Bacillus subtilis spores, these factors of resistance arequickly lost and germinated spores become sensitive to environmentalstress.Interestingly, C. perfringens spores can complete their germination inless than 20 min in the presence of L-asparagine and Kþ ions (AK). Since germinated spores are sensitive to heat and pressure, we hypothesized that formulating foods with certain germinants and then using HPP protocols that includea spore germination step may lead to an effective strategy to inactivatebacterial spores. Consequently, the objectives of thisstudy were to develop: (i) a germination-activation procedure forC. perfringens spores and (ii) thermal and HPP protocols for theinactivation of germinated spores of C. perfringens in poultry meat.
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