2.1.
Materials
All
chocolate
samples
used
in this
study
were
provided
by
Bel-
colade,
division
of
Puratos
(Industrielaan
16,
Industriezone
Zuid
III,
9320
Erembodegem,
Belgium).
Two
types
of
samples
were
ana-
lysed,
standard
and
artificially
off-flavoured, of
three
different
kind
of
chocolate;
dark,
milk
and
white.
Standard
samples
were
taken
from
the
conventional
chocolate
process
carried
out
at
industrial
scale.
All
the
samples
were
belong-
ing
to batches
produced
from
June
2012
to January
2013.
The
chocolate
samples
were
provided
in drops
(weight
∼3,20
g each)
or
as
tablet
(∼250
g each).
Upon
sampling,
they
were
stored
wrapped
up
in a double-layer
aluminium
foil
to avoid
direct
exposure
to light
and
oxidation
reactions
and
hermetically
packed
in high
barrier
plastic
bags
until
analysis.