Information about the effect of manuring on water quality and fecal contamination is scarce. Additionally the usual practice of fish pond owners to keep CM in tightly sealed plastic bags and leave it for some time in the sun, creates a condition resembling to some extent the anaerobic thermophilic fermentation process. So the aim of this study is to clarify the impact of using raw CM in fish farm environments on the occurrence of water pollution indicator bacteria, defining related potential health risks represented by pathogenic bacterial contamination especially the detection of Salmonella and E. coli O157.