An effective biosurfactant-producer and hydrocarbon degrading bacterial strain, Bacillus methylotrophicus
USTBa was isolated from hydrocarbon contaminated aqueous medium using crude oil as sole source of
carbon. The growth parameters such as pH, temperature, and salinity were optimized for degradation
of hydrocarbons and 92% degradation of crude oil removal was observed in 2 weeks. The biosurfactant
produced during the course of hydrocarbon degradation was monitored by surface tension and cell
hydrophobicity measurements. The produced biosurfactant had the ability to decrease the surface tension
of water from 72 to 28 mN/m, with the critical micelle concentration (CMC) of 35 mg/L. The biosurfactant
exhibited 90% emulsification activity (EI) on crude oil. Nuclear magnetic resonance spectroscopy
(NMR), X-ray diffraction (XRD), and thermal gravimetric (TG) analysis revealed the functional groups,
surface nature and thermostability of the biosurfactant, respectively. Gas chromatography analysis demonstrated
that the strain USTB efficiently degraded different alkanes from crude oil. The biosurfactant did
not exhibit inhibitory effect to various vegetables, however strong antibiotic activity against gram positive
and gram negative bacteria was observed. The study suggest application of the USTBa biosurfactant
as an appropriate candidate for bioremediation of crude oil contaminants.