The B. megaterium ACBT03-M3 survived in 10 g/l colchicine was having poor biomass yield as compare to control. However, the high concentration colchicine adapted bacterial cells were grown relatively lower amount 7 g/l of colchicine containing medium showed luxury growth as noticed in control conditions. On the basis of above results B. megaterium ACBT03-M3 was used
in subsequent experiments for production of 3-DMC and 3-DMTC.