Normal bile salt concentrations inside the inside
the small intestine is between 0.3 to 0.5%. However,
this concentration are directly related with the fat
intake and concentrations up to 3.0% could be found
(Kailassapathy and Chin 2011; Whitehead et al.,
2008). So, to better understand the bile tolerance
process, a longer time (24 h) was chosen because
it is expected than probotics survive adhered to the
intestine more time than the physiological transit
time, and the capacity of adaptation to the presence
of bile salts is related with some physiological
benefits like the serum cholesterol reduction (Haman
et al., 2011). However, the ileum and dudodeum
simulation (under normal conditions of bile salts and
pancreatin) was previously reported for these strains
showing a great bile salt tolerance under normal
physiological conditions (5 mg/ml bile salt plus 1.9
mg/ml pancreatin during 3 h)