The inhibitory effects of CFCS against the target microorganisms
were examined in Luria Bertani broth medium. For this, 20 ml of
CFCS was added to the 100 ml cultures of target microorganisms at
an early exponential phase (at 4 h). The optical density was
measured in triplicates at OD 600 at every hour. For negative control
target microorganism without CFCS added was used (Ghanbari,
Jami, Kneifel,&Domig, 2013)