Molecular analysis
Leaves from all the somaclones FR13A and FR13B were
collected and DNA isolated for molecular analysis prior to
screening for submergence tolerance. The DNA from
each genotype was PCR amplified and checked for the
presence of Pdc1 gene. It was seen that in 33 out of 45
clones, the primer amplified the pdc1 sequence from
FR13A-20 kr derived plants and 12 samples out of 25
were amplified from FR13A derived from 25 kr treatment
(Figure 4). When the amplification was done with
FR43B somaclones, only 23 out of 130 samples from
FR43B-20 kr treatment and only 9 out of 48 samples
derived from FR43B-25 kr treatments were amplified
(Figure 5). All the somaclones with the pdc1 were tolerant
to submergence irrespective of the radiation dose. This
may be clear to suggest that pdc1 gene is directly linked
to submergence in rice.