Different beef sausage samples were prepared for microbiological
analysis in accordance with ISO 6887-1 (2003), test
method of sample preparation (for microbiology of food
and animal feeding stuffs – Preparation of test samples, initial
suspension and decimal dilutions for microbiological
examination). Different beef sausage samples of 10 g were
weighed in the sterile stomacher zipped bag. Maximum
recovery solution (MRD), of 90 ml was added. The sample
and (MRD) solution were blended at low speed for 30–
60 s in stomacher machine. A dilution series were prepared
by transferring 1 ml of the previous dilution to 9 ml of
(MRD) solution. For detection of Salmonella sp., 25 g of
different sausage samples was weighed in the sterile stomacher
zipped bag, and 225 ml of buffered peptone water
(BPW) was added according to the reference ISO 6579
(2002).