the c17.2 cells have previously been used investigations of therapeutic transplantation in the treatment ofneurodegenerative disorder in mouse models. the objective of this project was to differentiate the cells into amixed culture of neurens and astrocytes in a controlled manner. previous studies have shown that the c17.2 cells secreate ngf and bdnf, but also glial cell line derived neurotrophic factor, stimulating autocrine induction of differentiation. indeed, just leaving the cells in complete dmem for 8days decreased the nestin expression and increase the expression of tubulin ad gfap.