Microbial growth and metabolism inevitably lead to a change in the hydrogen ion balance and, hence, the pH of the culture medium. Preliminary experiments showed that lipase production was highly sensitive to pH alteration during the fermentation. In all cases maximum activity was recorded at pH 5.59 -0.5 of the medium. The effect of initial pH of the medium was conducted at five different pH values varying from 4 to 8. The result is depicted in Fig. 3. The maximum lipolytic activity was recorded at pH(initial)6