The textile industry wastewater has been decolorized efficiently by the white rot fungus, Irpex lacteus,
without adding any chemicals. The degree of the decolorization of the dye effluent by shaking or sta-tionary cultures is 59 and 93%, respectively, on the 8th day. The higher level of manganese-dependent
peroxidase (MnP) and non-specific peroxidase (NsP) was detected in stationary cultures than in the cul-tures shaken. Laccase activities were equivalent in both cultures and its level was not affected signif-icantly by the culture duration. Neither lignin peroxidase (LiP) nor Remazol Brilliant Blue R oxidase
(RBBR ox) was detected in both cultures. The absorbance of the dye effluent was significantly
decreased by the stationary culture filtrate of 7 daysin the absence of Mn (II) and veratryl alcohol. In
the stationary culture filtrate, three or more additional peroxidase bands were detected by the zymo-gram analysis.