Background: Theanine, an amino acid in tea, has significant anti-stress effects on animals and humans.
However, the anti-stress effects of drinking green tea have not yet been elucidated.
Hypothesis/purpose: The present study aimed to explore anti-stress effects of green tea and roles of tea
components in a mouse model of psychosocial stress.
Study design: We examined anti-stress effects of three types of green teas, theanine-rich “Gyokuro”, standard
“Sencha”, and Sencha with lowered caffeine (low-caffeine green tea). Furthermore, the roles of tea
components such as caffeine, catechins, and other amino acids in anti-stress effects were examined.
Methods: To prepare low-caffeine green tea, plucked new tea leaves were treated with a hot-water spray.
Mice were psychosocially stressed from a conflict among male mice under confrontational housing. Mice
consumed each tea that was eluted with room temperature water ad libitum. As a marker for the stress
response, adrenal hypertrophy was compared with mice that ingested water.
Results: Caffeine was significantly lowered by spraying hot-water on tea leaves. While epigallocatechin
gallate (EGCG) is the main catechin in tea leaves, epigallocatechin (EGC) was mainly infused into water
at room temperature. Adrenal hypertrophy was significantly suppressed in mice that ingested theaninerich
and low-caffeine green tea that were eluted with water at room temperature. Caffeine and EGCG
suppressed the anti-stress effects of theanine while EGC and arginine (Arg) retained these effects.
Conclusion: These results suggest that drinking green tea exhibits anti-stress effects, where theanine,
EGC and Arg cooperatively abolish the counter-effect of caffeine and EGCG on psychosocial stress induced
adrenal hypertrophy in mice