2.3 Plant Analysis
Samples (0.10 g) from each treatment
were digested with 5.0 ml HNO3-H2O2 (4:1, v/v)
using a hot block heater. After cooling, the
digest was transferred to a 20-ml volumetric flask
and then filtered. Zn concentrations of these
samples were determined using inductively
coupled plasma mass spectrometry (ICP-MS;
Agilent Modell 7500a, Agilent Technologies,
USA).