Phosphorylases represent a wide range of highly flexible enzymes that can be used to synthesise, or phosphorolyse, a diverse range of carbohydrate structures. Significantly, this class of enzyme has already been utilised for the commercial synthesis of 2-O-α-d-glucosyl glycerol 4 and for the kilogram scale preparation of lacto-N-biose. 5 With the increasingly high speed and low cost of modern genome sequencing technologies, more of these enzymes are being discovered, which will add substantially to the toolbox of carbohydrate-active enzymes available for synthesis applications.