Methanogens were inhibited with BESA in the second enrich-
ment step at pH 7 with all pulp materials (Fig. S1). The hydrogen
yields with dry birch, dry conifer, wet birch and wet conifer were
68 ± 6, 76 ± 4, 66 ± 10 and 68 ± 7 mL H2/g TS, respectively. The
hydrogen production yields were lower than in the first enrich-
ment step, which may have been due to BESA inhibition of some
Clostridia [17]. Acetate was the main soluble metabolite, while
propionate concentration was lower than in the first enrichment
culture.
Methanogens were inhibited with BESA in the second enrich-ment step at pH 7 with all pulp materials (Fig. S1). The hydrogenyields with dry birch, dry conifer, wet birch and wet conifer were68 ± 6, 76 ± 4, 66 ± 10 and 68 ± 7 mL H2/g TS, respectively. Thehydrogen production yields were lower than in the first enrich-ment step, which may have been due to BESA inhibition of someClostridia [17]. Acetate was the main soluble metabolite, whilepropionate concentration was lower than in the first enrichmentculture.
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