2, 4-D is an herbicide and secondarily a plant growth regulator. It is used for broadleaf weed control. It has
little effect on grasses. It appears to work by causing uncontrolled cell division in vascular tissue. Abnormal increases in
cell wall plasticity, biosynthesis of proteins, and production of ethylene occur in plant tissues following exposure, and
these processes are responsible for uncontrolled cell division [11]. Methyl metsulfuron is a systemic and selective
herbicide; it can be used pre and post-emergence for control the broadleaf and sedges [14]. Move of action of this
herbicide is able to stop the plant cell division by blocking the action of the enzyme AcetoLactate Synthase (ALS) and
AcetoHydroxy Synthase (AHAS) to inhibit the conversion of α-ketoglutarate to 2 acetohydroxybutyrate and pyruvate into 2-acetolactate resulting acid chain branches amino valine, leucine, and isoleucine are not generated. Without these
essential amino acids, the protein can not be formed and lead to the plant die.