Vinegar solution: For the vinegar solution, 100 ml of vinegar was
measured. Then, 900 ml of water was added into vinegar, the
solution was stirred lightly to dissolve the solutions. If there was
residue present in the solutions, the solution was filtered and
supernatant was collected.
Preparation of simulated chlorpyrifos pollution in cauliflower
Preparation of chlopyrifos mixture: 2.0 L of water was added into
the 0.95 ml of chlorpyrifos. After water was added, the mixture was
stirred until dissolved.
Preparation of chlorpyrifos pollution in cauliflower: A cauliflower
of 1000 g was bought from the supermarket and washed with tap
water and dried with absorbent paper. Then, the cauliflower was
soaked in chlorpyrifos mixture for 10 min and stored at room
temperature in a dark room for 24 h