The rate of this promotion was recorded ~200٪ for node explants as well. Regarding root number, use of Active charcoal a critical so that control plantlet did not show any rooting activity in media lacking this agent. Since this phenomenon is necessary to transferring plantlets from invitro conducted to greenhouse/open field, apply a low cost agent in media used for micropropagation of stevia. This is accounted a cast effective method to produce high number of rooted plantlets without using any root inducing growth regulators.