The leaves of transgenic plants were collected and used to check
the presence of Cry1Ac protein using an immunostrip assay
(Bt-Cry1Ab/1Ac ImmunoStrip
test following
the manufacturer’s instructions. The leaf sample (80–100 mg
per plant) was ground using liquid nitrogen and crude protein
was extracted using 1.5 ml 1sample extraction buffer 4surface of the other leaf using a small brush. Then, the relative her-bicide tolerance of the plants was evaluated visually one week
after application.