been ฉันรักแปลThe tests were carried out in an Electrotek Anaerobic Workstation, where a microaerophilic environment was maintained (10% CO2 and 5% O2) at 37 °C. This would also test the effect of γ-PGA on the survival of bacteria when exposed to some oxygen just before ingestion. The cell concentration was measured at 0 h, 1 h, 2 h, 3 h and 4 h for γ-PGAimmobilised and free cell. This was done by making appropriate dilution of samples up to 10−8 and plating them out on BSM agar. Plates were incubated anaerobically at 37 °C for 48 h to assess the cell concentration at different incubation times in SGJ.