On completion of skin biometrics, five areas measuring about
5 cm2
were delineated on the volunteers’ forearms (one as a
control and four for testing the formulations). The formulation
containing free OMC was applied on the right forearm
and the liposome/OMC formulation was applied on the left
forearm. The formulations were administered with the aid of
a swab in an amount of approximately 10 mg. After defined
periods of time (15, 60, 120, and 240 minutes), the formulations
were removed with the aid of wet cotton. After drying
the formulation area, 11 tape strips measuring 1 cm2
were
sequentially applied to the skin and then quickly removed.
The first tape was discarded, and the other ten were submitted
to extraction with 90% ethanol. The OMC was then quantified
from the extraction solution by high-performance liquid
chromatography.3