Subsequently, after adding staining solutions into the reaction mixture, it was allowed to stand at 80°C for 10 min, and measurement with a T80 UV/VIS Spectrophotometer (PG Instruments Ltd., Leicestershire, UK) was performed against
blind sample that was created by putting distilled water, and activity was determined by monitoring absorbance change at 540 nm (Senol et al. 2014).
Subsequently, after adding staining solutions into the reaction mixture, it was allowed to stand at 80°C for 10 min, and measurement with a T80 UV/VIS Spectrophotometer (PG Instruments Ltd., Leicestershire, UK) was performed againstblind sample that was created by putting distilled water, and activity was determined by monitoring absorbance change at 540 nm (Senol et al. 2014).
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