In addition, there was no effect of the BV and melittin on the anxiety parameters assessed by the elevated plus-maze test. It can indicate an absence of interaction with GABAergic system. Diazepam has been employed in behavioral pharmacology as a reference compound for potential anxiolytic-like substances (Mizushige et al., 2013). Here, it increased the number of entries in the open arms as expected. Although diazepam promoted an increase about 30% on the percent of the time spent in the open arms from the saline group, the statistical analysis failed to reveal a significant effect. This may be due the high SEM in the control groups and/or the reduced number of animals in some groups (n = 5-9).