1. You will need a bottle of blue dye.
2. Prepare the following dilution blanks with tap water using a 10ml pipette and the green pi-pump---4.5ml, 9ml, 9.5ml, 4ml, 3ml, 12ml.
3. Make 3 sets of dilution tubes as seen in the accompanying diagram. Mix the tube contents.
Dilution set 1: Transfer 0.5ml of blue water into the 4.5ml of water, then 1ml of tube 1 into the next tube of 9ml water.
Dilution set 2: Transfer 1ml of blue water into the 4ml of water, then 0.5ml of tube 1 into the next tube of 9.5ml.
Dilution set 3: Transfer 1ml of blue water into the 3ml of water, then 0.5ml of tube 1 into the next tube of 12ml.
4. Interpretation of your dilution tubes--a. Record the dilution values of your tubes in the below. b. Check the last tubes of each set against each other to make sure that they are the same shade of blue