Commonly used medicines that are often involved in court litigations related with driving under the influence of alcohol were selected.
Alprazolam, flunitrazepam and tramadol were tested as drugs with no known effect on ADH activity.
Cimetidine, reported previously as having inhibitory effect on ADH, and 4-methylpyrazole (4-MP),
a well known ADH inhibitor, were tested as positive controls