Keratinases and gelatinase assays
Keratinase assay was done as described by Mazotto et al.
[14]. One unit of keratinolytic activity was defined as the amount of enzyme required to produce an increase of
0.01 absorbance unit, at 280 nm, under standard assay
conditions (1 h at 37°C). Gelatinases were analyzed
according the method described by Cedrola et al [16]
One unit of gelatinase activity was defined as the
amount of enzyme required to produce 1 lg of peptides
under the described assay conditions.