The isolates were previously grown on autoclaved rice during
nine days in Petri dishes and further added to a commercial
substrate (Baseplant) at 72 mg.kg-1, in pots (1 L) (Barbosa et al.,
2010). Three peanut seeds previously surface-sterilized
(hypochlorite solution at 1.5%) were sown in each pot and daily
watered. Two earliness-upright cultivars were used in this assay:
Senegal 55 437, a Spanish type developed by International Crops
Research Institute for the Semi-Arid-Tropics (ICRISAT), and BR 1, a
Valencia type developed by Brazilian Company of Agricultural
Research (EMBRAPA). Taking in account that the low fertility of
substrate could affect the fungus pathogenicity, this assay was
repeated in the same conditions with supplementation of 40 g P2O5,
15 g KCl and 200 g hummus, added to each kg substrate, based on
recommendations in Santos et al. (2006). The experimental design
was completely randomized with eight replications.