Samples were passed through a Sep-Pak C18 cartridge (Waters,Milford, MA, USA) and retained compounds were eluted with 60% acetonitrile containing 0.1% TFA in water. The antioxidant capacity of samples was measured in duplicate by fluorescence using the ORAC method as described previously (Torino et al., 2013). Results were expressed as lmol Trolox equivalents (TE)/g hydrolysate.