Conclusions This study successfully develop the HPLC method for the crude extract of Zingiber cassumunar Roxb., especially for that of the compound D. The mobile phase comprised of 2% acetic acid in deionized water and methanol with gradient elution in 35 minutes. This was significantly shorter compared to other publications that reported more than 110 minutes. In addition, the retention time of compound D was reduced to 6.42 minutes. This method showed good linearity, accuracy, and precision. Compound D could be clearly separated from the various Thai herbs in the Thai Herbal Compress ball. Therefore, this successfully developed HPLC method can be used for future study of different Thai Herbal Compress ball formulations.