Maceration extraction is extract that rely on the spread of solution into the matrix of the sample. Often use this method with high temperature to increase efficiency , The tissue samples will weaken in to solution matrix of the sample and soluble compounds in the sample into the solution , besides this method used a long time and high temperature effected to the nature of compound not stability to heat or oxygen ,the result oil extraction from papaya seed by maceration have a good condition at temperature 57.51 °C , time 9 hr, and sample to solvent ratio 1:20 v/w , that is a good optimizasion and gave yield 29.14 %