TSA dishes were streaked with B. linens IC10 or B. subtilis on one half of each dish. The dishes were incubated for 24 h at 26 °C. Agar plugs containing actively growing mycelium of B. cinerea were placed in the center of the remaining half of each dish. The dual confrontation culture dishes were incubated for an additional 48 h at 26 °C. Following the confrontation period, the TSA containing the bacteria and the visible inhibition zone was recovered, cut into cubes, lyophilized, and ground to a powder.