The bacterial C1AC55.07 strain used was isolated from sugarcane cultivated soil at the Japungu Agroindustrial S.A. located in the Santa Rita city (Brazil) and classified as Bacillus sp. by 16S rDNA sequence analysis. The bacterial strain was evaluated for enzyme production of carboxymethylcellulase (CMCase) using carboxymethylcellulose (CMC)-containing agar medium and incubation at 55°C for 3 days. Cellulolytic activity was detected by the formation of clear zones around colonies through the Congo red staining.