In the present paper, rubber seed oil (RSO) has been investigated as a potential source for biodiesel production
in Bangladesh. Rubber seed oil has been extracted from the rubber seeds collected from the local
garden. Different methods have been applied for the oil extraction, such as mechanical press with and
without solvent and cold percolation. Maximum oil content of 49% has been found by mechanical press
with periodic addition of solvent. The physico-chemical properties of the oil have been investigated.
Effect of seed storage time on free fatty acid (FFA) content of the oil is studied an it is found that the
FFA content increases from 2 wt.% (fresh seed) to 45 wt.% after 2 months of storage at room temperature.
Biodiesel has been prepared using a three-step method comprises with saponification of oil, acidification
of the soap and esterification of FFA. Overall yield of FFA from RSO is found to be around 86%. The final
step is esterification that produces fatty acid methyl ester (FAME). The effect of methanol to oil ratio and
catalyst content has been investigated for esterification reaction. 1H NMR spectrum of the RSO and biodiesel
samples are analyzed which confirms the conversion of RSO to biodiesel. The biodiesel properties
have been investigated and are found to be comparable with diesel.